Existence of Paenibacillus larvae genotypes ERIC I-ST2, ERIC I-ST15 and ERIC II-ST10 in the western region of Aichi prefecture, Japan
نویسندگان
چکیده
American foulbrood is the most destructive honeybee bacterial disease. The etiological agent, Paenibacillus larvae, has been classified into four genotypes by a repetitive-element PCR (ERIC I-IV) and 21 sequence types by multilocus sequence typing (ST1-21). In this study, we genotyped Japanese P. larvae isolates for the first time and revealed the presence of three genotypes (ERIC I-ST2, ERIC I-ST15 and ERIC II-ST10) in the western region of Aichi prefecture. ERIC I-ST15 and ERIC II-ST10 are globally distributed types, whereas the ERIC I-ST2 isolate was the first isolate of this genotype identified outside the native range of the European honeybee. The ERIC I and II isolates differed in phenotypes including cell morphology, and these may be useful for predicting ERIC types.
منابع مشابه
Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
The gram-positive, spore-forming bacterium Paenibacillus larvae is the etiological agent of American Foulbrood (AFB), a globally occurring, deathly epizootic of honey bee brood. AFB outbreaks are predominantly caused by two genotypes of P. larvae, ERIC I and ERIC II, with P. larvae ERIC II being the more virulent genotype on larval level. Recently, comparative proteome analyses have revealed th...
متن کاملMultiple Locus Variable number of tandem repeat Analysis: A molecular genotyping tool for Paenibacillus larvae
American Foulbrood, caused by Paenibacillus larvae, is the most severe bacterial disease of honey bees (Apis mellifera). To perform genotyping of P. larvae in an epidemiological context, there is a need of a fast and cheap method with a high resolution. Here, we propose Multiple Locus Variable number of tandem repeat Analysis (MLVA). MLVA has been used for typing a collection of 209 P. larvae s...
متن کاملCharacterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection.
Paenibacillus larvae is the causative agent of American Foulbrood (AFB), the most severe bacterial disease that affects honeybee larvae. AFB causes a significant decrease in the honeybee population affecting the beekeeping industry and agricultural production. After infection of larvae, P. larvae secretes proteases that could be involved in the pathogenicity. In the present article, we present ...
متن کاملNegative correlation between individual-insect-level virulence and colony-level virulence of Paenibacillus larvae, the etiological agent of American foulbrood of honeybees.
Paenibacillus larvae is the etiological agent of American foulbrood (AFB) in honeybees. Recently, different genotypes of P. larvae (ERIC I to ERIC IV) were defined, and it was shown that these genotypes differ inter alia in their virulence on the larval level. On the colony level, bees mitigate AFB through the hygienic behavior of nurse bees. Therefore, we investigated how the hygienic behavior...
متن کاملMultidrug-Resistant Strains of Acinetobacter baumannii from Intensive Care Unit Patients Show Genetic Diversity and Distribution of Genes Associated with Aminoglycoside Resistance
Background and Objective: As a significant cause of nosocomial infections, Acinetobacter baumannii has been linked to opportunistic infections. The assessment of clonal relatedness of A. baumannii isolates using typing methods like ERIC-PCR is beneficial for controlling conditions due to these resistant isolates. This research aims to study Acinetobacter baumannii resistant isolates to multidru...
متن کامل